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Extension of SP1 primer by proofreading T7 DNAP. The positions of unextended (inverted open triangle) and extended (inverted black triangle) primers are indicated. All spectra contain one additional equivalent of primer as a post-reaction internal standard. (A–D) Correct primer–substrate pairs. (A) LTT + dATP. (B) LTA + dTTP. (C) LTG + dCTP. (D) LTC + dGTP. (E–H) Incorrect primer–substrate pairs. (E) LTG + dATP. (F) LTC + dTTP. (G) LTA + dCTP. (H) LTA + dGTP.
