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SBE reactions of Vent DNAP variants with dideoxy- and acyclonucleotide terminators. The positions of unextended (inverted open triangle) and extended (inverted black triangle) primers are indicated. (A–C) Primer extension for LTC/ddTTP. (A) P1/exo– Vent. (B) SP1/exo– Vent. (C) SP1/exo+ Vent. (D–F) Primer extension for LTT/acyCTP. (D) P1/exo– Vent. (E) SP1/exo– Vent. (F) SP1/exo+ Vent.
