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Primer extension under limiting substrate concentrations. LTG template with dCTP: 2.5 µM (inverted black triangle), 10 µM (open circle), 40 µM (filled square) and 160 µM (open triangle). (A) 1 U Sequenase 2.0. (B) 1 U exo+ T7 DNAP. (C) 0.25 U Sequenase 2.0. (D) 0.25 U exo+ T7 DNAP.
