Table 1. Primer extension with dNTP substrates.

Template	Substrate	Primer extension by dNTP (%)
	Polymerase	KF–	KF–	KF+	T4+	T7–	T7+	V–	V–	V+
	Primer	P1a	SP1a	SP1a	SP1a	P1a	SP1a	P1b	SP1b	SP1b
LTA	dATP	69	53	17	6	23	–	13	12	–
	dCTP	38	23	–	–	7	–	–	–	–
	dGTP	–	–	–	–	–	–	–	–	–
	dTTP	94	88	86	79	81	63	100	91	81
LTC	dATP	52	43	9	–	15	–	26	12	–
	dCTP	–	–	–	–	–	–	–	–	–
	dGTP	100	100	100	100	93	81	100	85	81
	dTTP	–	–	–	–	–	–	64	32	13
LTG	dATP	19	15	–	–	–	–	–	–	–
	dCTP	100	100	100	100	100	88	100	81	75
	dGTP	48	35	11	–	16	–	17	15	–
	dTTP	24	18	11	–	28	–	37	16	–
LTT	dATP	92	90	86	73	82	67	100	84	76
	dCTP	20	17	6	–	–	–	19	9	–
	dGTP	74	67	21	8	13	–	25	17	7
	dTTP	–	–	–	–	–	–	38	11	–
	Incorrect average	29	23	6	1	9	0	20	10	2
	Correct average	97	95	93	88	89	75	100	85	78
	I/Cavg (%)	30	24	7	1	10	0	20	12	2

Exonuclease-deficient (–) and proofreading (+) variants of Klenow fragment (KF), T4 DNAP (T4), T7 DNAP (T7) and Vent DNAP (V) are indicated. Correct nucleotide incorporation for each template is shown in bold. – entries indicate values below the limit of detection (<5%).

^a10 µM primer, 25 µM template, 200 µM substrate, 1 U polymerase, 60 min at 37°C.

^b10 µM primer, 4 µM template, 200 µM substrate, 1 U polymerase, 25 cycles of 30 s at 85°C, 1 min at 53°C, 1 min at 63°C.