Table 3. Primer extension with acyNTP substrates.

Template	Substrate	Primer extension by acyNTP (%)
	Polymerase	KF–	KF–	KF+	T4+	T7–	T7+	V–	V–	V+
	Primer	P1a	SP1a	SP1a	SP1b	P1b	SP1b	P1c	SP1c	SP1c
LTA	acyATP	10	10	–	–	–	–	–	–	–
	acyCTP	–	–	–	–	–	–	–	–	–
	acyGTP	–	–	–	–	–	–	8	–	–
	acyTTP	64	56	59	78	81	82	74	64	62
LTC	acyATP	–	–	–	–	–	–	9	–	–
	acyCTP	14	9	–	–	–	–	–	–	–
	acyGTP	100	100	100	100	100	100	100	100	100
	acyTTP	–	–	–	–	–	–	29	20	–
LTG	acyATP	–	–	–	–	–	–	–	–	–
	acyCTP	100	100	100	100	100	93	100	100	100
	acyGTP	12	11	–	–	39	–	45	28	–
	acyTTP	–	–	–	–	–	–	42	26	–
LTT	acyATP	100	100	100	92	93	86	79	68	69
	acyCTP	10	–	–	–	–	–	42	34	–
	acyGTP	15	12	–	–	–	–	23	10	–
	acyTTP	–	–	–	–	–	–	37	34	–
	Incorrect average	5	4	0	0	3	0	20	13	0
	Correct average	91	89	90	93	94	90	88	83	83
	I/Cavg (%)	6	4	0	0	3	0	22	15	0

Polymerase identities are the same as those listed for Tables 1 and 2. – entries indicate data below the limit of detection (<5%).

^a10 µM primer, 25 µM template, 500 µM substrate, 2 U polymerase, 4 h at 37°C.

^b10 µM primer, 25 µM template, 500 µM substrate, 2 U polymerase, 6 h at 37°C.

^c10 µM primer, 4 µM template, 200 µM substrate, 2 U polymerase, 35 cycles of 30 s at 85°C, 1 min at 53°C, 1 min at 63°C.