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Addition of VAMP-7 to in vitro homotypic/heterotypic endosome and lysosome (Lyso) fusion. Specified amounts of recombinant VAMP-7 (minus the transmembrane domain) were added to the in vitro fusion reaction of E4–E4, E8–E8, E12–E12, Lyso–Lyso (all homotypic), and E12–Lyso (heterotypic). The data are expressed as the percentage of fusion, with 100% fusion representing the maximum fusion observed for each vesicle population.
