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. 2003 Feb 1;31(3):e10. doi: 10.1093/nar/gng010

Figure 1.

Figure 1

(A) Structure of the novel three (ZFAV) and five (5ZFAV) zinc finger transcription factors binding to their cognate sites in the bax promoter. ZF1 and ZF2 are wild-type zinc fingers of the transcription factor Zif268. ZF1* and ZF3* are zinc fingers selected by screening combinatorial libraries (22). VP16 is a trans-activation domain and NLS is a nuclear localization sequence. The consensus p53 binding site is shown in the box and the zinc finger binding sites are shown in bold. (B) Protein expression. Saos-2 nuclear cell extracts were immunoblotted with anti-Myc (9E10) antibody. The black arrows show the positions of the zinc finger proteins; the gray arrowhead marks non-specific bands seen in controls.