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. 2003 Feb 1;31(3):e11. doi: 10.1093/nar/gng01

Figure 2.

Figure 2

PCR products checked on 1% agarose gel. (A) Single-stranded template was amplified by linear PCR (lane 1), after mutant strand synthesis, primers PCR5′ and PCR3′ amplified the mutant strand (lane 3) rather than the wild-type plasmid (lane 4); the same is true with primers MutPCR1 and MutPCR2 (lanes 5 and 6). (B) PCR product shown in lane 3 of (A) was cloned into pGEM-T Easy and bacteria transformed. The mutant primers PCRMut1 and PCRMut2 amplified all of the 16 white colonies selected (1–16), but not the wild-type vector (–c).