Table 1. Nucleotide sequence of the primers used, with the mutation points shown in lower case.

Name	Sequence
Anchor5′	5′-CGAATTCCCGgtaCCGCCA-3′
Anchor3′	5′-TGCAGGCGGCCGCGAgTcgACTAGT-3′
Mut1	5′-TAAGAAGATCaCTATGCCC-3′
Mut2	5′-GCATGCTACTaAGCTTTCA-3′
PCR5′	5′-CCGGCCGCCATGGCGGtac-3′
PCR3′	5′-TGCAGGCGGCCGCGAgTcg-3′
MutPCR1	5′-GGCAACTCAAGGGCATAGt-3′
MutPCR2	5′-GCTCTGAGCATGCTACTa-3′