Figure 2.

skn7Δ cells are sensitive to acute heat stress. Midlog-phase cultures of W303-1a and isogenic skn7Δ cells were grown in YPD at 25°C, and an aliquot was shifted to a test tube placed in a 51°C water bath. Samples were taken at the times indicated, diluted into ice-cold YPD, and immediately plated onto YPD agar to assess cell viability. Survival at 51°C was expressed as a percentage of viable cells relative to cells grown at 25°C. Because the vertical axis is logarithmic, only positive errors are included for clarity.