Figure 5.

Genetic interactions between SKN7 and HSF1. (A) Single-copy expression of SKN7 rescues the growth defect of DR20-2b (hsf1^ts skn7Δ) at 33°C. DR20-2b (hsf1^ts skn7Δ) was transformed with the CEN vector YCplac111 (vector) or with this vector containing the SKN7 gene (+ SKN7) and was incubated at permissive (25°C) and intermediate (33°C) temperatures on rich medium. (B) High-copy expression of SKN7 rescues the growth defect of the hsf1^ts strain, MYY385, at 35°C. The hsf1^ts strain MYY385 was transformed with the 2μ-based plasmid YEp24 containing the SKN7 gene or with the vector alone (vector) and was streaked onto selective medium and incubated at 35°C for 4 d. The wild-type strain MYY290 (HSF1⁺) was included as a positive growth control.