Table 1.

Induction of SSA1-LacZ in response to t-butyl hydrogen peroxide requires Skn7

Strain	Hydrogen peroxide (0.6 mM)		Heat shock
	−	+	25°C	37°C
W303-1a	0.08	0.61	0.15	1.33
W303-1a skn7Δ	0.04	0.03	0.02	0.23

Wild type and isogenic skn7Δ cells containing pZJHSE2-137 were assayed for β-galactosidase levels before (−) and after (+) the addition of t-butyl hydrogen peroxide to 0.6 mM for 1 h. For the heat shock experiment, cells were grown at 25°C and transferred to 37°C for 1 h. β-Galactosidase activity is expressed as change in OD₄₂₀ per minute per milligram of protein. Values are averages of duplicate samples from two independent experiments.