Table 1.
Effect of I3C alone and in combination with putrescine on intracellular polyamine levels at 24, 96 and 168 hours.
| Treatment | Cell no. as % Control (SD) | Putrescine (SD) | Spermidine (SD) | Spermine (SD) |
| 24 hour | ||||
| Control | 100.0 (0.0) | 0.48 (0.31) | 1.62 (0.55) | 2.10 (0.98) |
| I3C 100 | 89.1 (19.8) | 0.31 (0.09) | 1.79 (0.51) | 2.25 (1.21) |
| I3C 175 | 87.9 (29.2) | 0.23 (0.06)* | 1.32 (0.36) | 1.54 (0.19) |
| I3C 250 | 77.4 (32.7) | 0.19 (0.04)* | 2.17 (0.98) | 2.73 (1.86) |
| I3C 500 | 54.2 (26.6) | 0.14 (0.07)* | 1.57 (0.60) | 2.74 (1.82) |
| C + P20 | 96.9 (23.5) | 0.64 (0.26) | 2.17 (1.06) | 2.41 (1.53) |
| C + P50 | 90.0 (42.1) | 0.72 (0.27)* | 2.34 (1.48) | 2.59 (1.93) |
| I + P20 | 76.2 (26.3) | 0.69 (0.29) | 2.93 (1.57)* | 3.06 (1.99) |
| I + P50 | 76.1 (35.0) | 0.54 (0.16) | 1.86 (0.49) | 1.94 (0.65) |
| 96 hour | ||||
| Control | 100.0 (0.0) | 0.08 (0.04) | 0.79 (0.30) | 1.25 (0.38) |
| I3C 100 | 98.7 (8.5) | 0.13 (0.09) | 1.11 (0.40) | 1.87 (0.47) |
| I3C 175 | 58.9 (3.8) | 0.13 (0.10) | 0.98 (0.29) | 2.18 (0.79)* |
| I3C 250 | 24.9 (6.1) | 0.15 (0.11) | 0.77 (0.36) | 2.03 (1.17)* |
| I3C 500 | 13.9 (6.0) | nd | 0.62 (0.32) | 1.34 (0.45) |
| C + P20 | 96.6 (12.4) | 0.13 (0.03) | 0.84 (0.15) | 1.15 (0.12) |
| C + P50 | 79.2 (14.3) | 0.32 (0.18)* | 1.47 (0.15)* | 1.98 (0.34) |
| I + P20 | 28.1 (22.6) | 0.17 (0.09) | 1.25 (0.23)* | 2.42 (0.21)* |
| I + P50 | 17.9 (2.9) | 0.11 (0.02) | 0.80 (0.21) | 1.54 (0.48) |
| 168 hour | ||||
| Control | 100.0 (0.0) | 0.04 (0.02) | 0.46 (0.07) | 0.76 (0.17) |
| I3C 100 | 104.0 (9.9) | 0.03 (0.01) | 0.57 (0.25) | 1.22 (0.32) |
| I3C 175 | 57.1 (9.5) | 0.05 (0.03) | 0.62 (0.28) | 1.99 (0.96)* |
| I3C 250 | 31.1 (20.2) | 0.07 (0.01) | 0.87 (0.54) | 2.93 (0.72)* |
| I3C 500 | 4.5 (5.2) | nd | nd | nd |
| C + P20 | 107.2 (4.8) | 0.03 (0.01) | 0.48 (0.04) | 0.83 (0.15) |
| C + P50 | 104.5 (10.1) | 0.06 (0.04) | 0.67 (0.10) | 1.08 (0.19) |
| I + P20 | 6.0 (5.5) | nd | 0.91 (0.55) | 3.11 (1.59)* |
| I + P50 | 3.0 (0.7) | nd | 0.90 (0.56) | 2.26 (1.42)* |
Cells were treated with I3C alone or in combination with putrescine, and intracellular polyamines determined as described in the Methods Section. Data shown for cell numbers are expressed as a % Control, and polyamine levels are nmol/106 cells. All values are means with SD in brackets. nd = values not determined. * indicates a value that is significantly different to the control value at that time point.