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Kar3p and Cik1p are tightly associated. Lysates from an untagged strain (Y1861; UT) or a KAR3::HAT strain (Y1870) were treated with final concentrations of 0.1, 1, or 2 M NaCl, 1 or 2 M urea, or 1% SDS. Lysates were then diluted and immunoprecipitated with anti-HA antibodies. Immunoblot analysis with anti-HA antibodies (top blot) and anti-Cik1p antibodies (bottom blot) were used to detect coimmunoprecipitation of Kar3-HATp and Cik1p from these treated lysates.
