Figure 7.
Kar3-HATp coimmunoprecipitates with Cik1p in vegetatively growing cells. Proteins from lysates of vegetatively growing cells from either untagged strains (wild type [Y1861] and cik1Δ [Y1850]) or KAR3::HAT strains (wild type [Y1870] and cik1Δ [Y1874]) were separated by SDS-PAGE and analyzed by immunoblotting (Lysates) or first immunoprecipitated with anti-HA or anti-Cik1p antibodies (IP Antibody). The genotype of the strain in each lane is denoted under the immunoblot. Immunoblots were probed with anti-HA antibodies.