Table 2.
Relative β-galactosidase activity of two-hybrid interactions
| lexA-DBD fusion | GAL4-AD fusion | Relative β-galactosidase activitya |
|---|---|---|
| Vector | Vector | 1.0 |
| Vector | Cik1(1–594)b | 0.5 |
| Vector | Kar3(12–514) | 0.5 |
| Vector | Kar3(104–236) | 1.7 |
| Vector | Kar3(176–294) | 1.5 |
| Vector | Kar3(238–408) | 1.1 |
| Cik1(20–541) | Vector | 0.5 |
| Cik1(20–541) | Cik1(1–594) | 0.9 |
| Cik1(20–541) | Kar3(12–514) | 34.1 |
| Cik1(20–541) | Kar3(104–236) | 26.8 |
| Cik1(20–169) | Vector | 0.7 |
| Cik1(20–169) | Cik1(1–594) | 0.8 |
| Cik1(20–169) | Kar3(12–514) | 85.4 |
| Cik1(20–169) | Kar3(104–236) | 23.7 |
| Cik1(20–169) | Kar3(176–294) | 1.9 |
| Cik1(20–169) | Kar3(238–408) | 1.2 |
| Cik1(20–147) | Vector | 1.1 |
| Cik1(20–147) | Kar3(12–514) | 51.2 |
| Cik1(20–130) | Vector | 1.0 |
| Cik1(20–130) | Kar3(12–514) | 0.8 |
| Cik1(20–114) | Vector | 0.9 |
| Cik1(20–114) | Kar3(12–514) | 0.8 |
| Cik1(77–207) | Vector | 0.7 |
| Cik1(77–207) | Kar3(12–514) | 37.8 |
| Cik1(77–169) | Vector | 1.1 |
| Cik1(77–169) | Kar3(12–514) | 19.0 |
| Cik1(77–169) | Kar3(104–236) | 92.7 |
| Cik1(77–169) | Kar3(176–294) | 21.2 |
| Cik1(77–169) | Kar3(238–408) | 17.3 |
| Cik1(207–368) | Vector | 0.8 |
| Cik1(207–368) | Kar3(12–514) | 63.4 |
| Cik1(207–368) | Kar3(104–236) | 2.2 |
| Cik1(207–368) | Kar3(176–294) | 24.4 |
| Cik1(207–368) | Kar3(238–408) | 212.2 |
| Cik1(218–393) | Vector | 0.7 |
| Cik1(218–393) | Kar3(12–514) | 14.6 |
| Cik1(218–393) | Kar3(104–236) | 0.6 |
| Cik1(218–393) | Kar3(176–294) | 0.9 |
| Cik1(218–393) | Kar3(238–408) | 34.1 |
a
These values are relative to those measured for the vector-vector interaction, which was normalized to a value of 1.0, and were averaged from two experiments.
b
Numbers in parentheses represent positions within the primary amino acid sequences of Cik1p and Kar3p encoded by each construct.