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Regulation of MT1-MMP–dependent subjacent proteolytic activity by α1PDX. Control-transfected (A and B), MT1-MMP–transfected (C and D), and MT1-MMP– and α1PDX–cotransfected (E and F) CHO-K1 cells were cultured on surfaces coated with Texas red–labeled gelatin. After 16 h of incubation, cells were visualized by phase-contrast microscopy (A, C, and E), and zones of gelatinolytic activity were identified by confocal laser microscopy (B, D, and F).
