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Ribosome-inhibitory activity of PAP wild-type and mutants in in vitro rabbit reticulocyte lysate system. Each value is the average of three independent experiments. Protein synthesis was determined by measurement of [35S]methionine incorporation, and the level of incorporation for samples treated with all reagents except PAP was assigned a value of 100%. (A) FLP-102 and single-residue mutants FLP-100 and FLP-101. (B) FLP-105 and single-residue mutants FLP-103 and FLP-104.
