TABLE 1.
Sequence identities and biological activities of wild-type and mutant PAPs
| Mutant | Original residue | Substituted residue | IC50 (ng/ml) for translation inhibitiona | IC50 (ng/ml) of FLP-WT |
|---|---|---|---|---|
| FLP-WT | 3.0 (2.5-3.8) | 1 | ||
| FLP-100 | K151 | A151 | 147 (130-167) | 49 |
| FLP-101 | I152 | A152 | 617 (500-710) | 206 |
| FLP-102 | 151KI152 | 151AA152 | 994 (878-1,121) | 331 |
| FLP-103 | F191 | A191 | 57 (52-60) | 19 |
| FLP-104 | N192 | G192 | 54 (44-66) | 18 |
| FLP-105 | 191FN192 | 191AG192 | 105 (94-131) | 35 |
a
Each value is the mean of at least three experiments. The values in parentheses are 95% confidence limits.