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. 2000 Jul;11(7):2419–2428. doi: 10.1091/mbc.11.7.2419

Figure 4.

Figure 4

Long-term nucleolar localization of U6 snRNA. The nucleolar localization of U3 snoRNA, U6 snRNA, U2 snRNA, and a 40-nt control RNA was analyzed 24 h after injection into oocyte nuclei. U3 snoRNA strongly localizes to nucleoli. In contrast to earlier time points, nucleoli are only weakly stained by U6 snRNA (with capped U6 snRNA, signals were slightly more variable than with all other transcripts and occasionally moderate nucleolar staining was observed). As with earlier time points (Figures 1–3), U2 signals remain weak in nucleoli, and nucleoli are not stained by the control RNA. B-snurposomes (which are DAPI-negative) are stained by U2 snRNA at this time point. Other details as in Figure 1.