TABLE 3.
Presence of vanA or vanB mRNAs (as detected by RT-PCR) in 7-, 14-, and 28-day-old VBNC cells from clinical and veterinary VRE isolatesa
| Strain | Genotype | mRNA from cells maintained in the VBNC state for:
|
||
|---|---|---|---|---|
| 7 days | 14 days | 28 days | ||
| Noninduced E. faecalis V583 | vanB | −b | − | NDe |
| Induced E. faecalis V583 | vanB | +c | + | + |
| E. faecalis EFV1 | vanA | + | + | + |
| E. faecalis VREa-13 | vanA | + | + | + |
| E. faecium EFAV-3 | vanB | + | + | ±d |
| E. faecium EFAV-15 | vanA | ± | − | − |
| E. faecium VREa-36 | vanA | + | ± | − |
Experiments were performed in triplicate.
−, absence of amplification products in all experiments.
+, presence of the amplification products in three out of three experiments.
±, presence of the amplification products in one or two out of three experiments.
ND, not determined.