Figure 6.

Intracellular localization of Luv1p. (A) GFP-Luv1p does not colocalize with Pep12p in wild type or vps27 mutants. Fixed wild-type (YMC6) or vps27 (YMC7) cells expressing GFP-Luv1p were immunostained for Pep12p and visualized for GFP (green) or Pep12p (red) in separate channels. Overlays of the separate images (merge) and Nomarski differential interference contrast images are also shown. (B) HA-Luv1p and Kex2p colocalize. Wild-type cells expressing HA-Luv1p and overexpressing Kex2p (YMC8) were immunostained for HA (green) and Kex2p (red) and visualized as in A.