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Comparison of expression of OprM homolog genes in wild-type PAK (H911) (top panel) and the opmG mutant (H958) (bottom panel) by using mini-microarrays. RNA isolated from each strain was reverse transcribed to cDNA, radiolabeled with [α-32P]dCTP, and hybridized to a nylon membrane containing 10-ng spots of 600-bp internal fragments corresponding to each of the OprM homologs. Spot densitometry revealed an absence of oprN expression as well as the expected absence of opmG expression.
