TABLE 1.
Description of primers used to detect each of the tetracycline resistance genes
| Group and PCRa | tet genes | Amplicon size (bp) | Annealing temp (°C) | Reference |
|---|---|---|---|---|
| Group 1, multiplex PCR | tet(B) | 659 | 55°C | 20 |
| tet(C) | 418 | 55°C | 20 | |
| Group 2, multiplex PCR | tet(A) | 210 | 55°C | 20 |
| tet(E) | 278 | 55°C | 20 | |
| Group 3, multiplex PCR | tet(K) | 169 | 55°C | 20 |
| tet(M) | 406 | 55°C | 20 | |
| tet(O) | 515 | 55°C | 20 | |
| tet(S) | 667 | 55°C | 20 | |
| Individual PCR | tet(L) | 267 | 55°C | 20 |
| Individual PCR | tet(Q) | 904 | 55°C | 20 |
| Individual PCR | tet(T) | 169 | 46°C | 1 |
| Individual PCR | tet(W) | 168 | 64°C | 1 |
a
A multiplex PCR was used for groups 1, 2, and 3; and an individual PCR was used for tet(Q), tet(T), and tet(W).