TABLE 2.
PCR primers targeting tet genes
| Primer | Class targeted | Sequence | Reference |
|---|---|---|---|
| TetA-FW | tet(A) | GCT ACA TCC TGC TTG CCT TC | 20 |
| TetA-RV | tet(A) | CAT AGA TCG CCG TGA AGA GG | 20 |
| TetB-FW | tet(B) | TTG GTT AGG GGC AAG TTT TG | 20 |
| TetB-RV | tet(B) | GTA ATG GGC CAA TAA CAC CG | 20 |
| TetC-FW | tet(C) | CTT GAG AGC CTT CAA CCC AG | 20 |
| TetC-RV | tet(C) | ATG GTC GTC ATC TAC CTG CC | 20 |
| TetE-FW | tet(E) | AAA CCA CAT CCT CCA TAC GC | 20 |
| TetE-RV | tet(E) | AAA TAG GCC ACA ACC GTC AG | 20 |
| TetK-FW | tet(K) | TCG ATA GGA ACA GCA GTA | 20 |
| TetK-RV | tet(K) | CAG CAG ATC CTA CTC CTT | 20 |
| TetL-FW | tet(L) | TCG TTA GCG TGC TGT CAT TC | 20 |
| TetL-RV | tet(L) | GTA TCC CAC CAA TGT AGC CG | 20 |
| TetM-FW | tet(M) | GTG GAC AAA GGT ACA ACG AG | 20 |
| TetM-RV | tet(M) | CGG TAA AGT TCG TCA CAC AC | 20 |
| TetO-FW | tet(O) | AAC TTA GGC ATT CTG GCT CAC | 20 |
| TetO-RV | tet(O) | TCC CAC TGT TCC ATA TCG TCA | 20 |
| TetS-FW | tet(S) | CAT AGA CAA GCC GTT GAC C | 20 |
| TetS-RV | tet(S) | ATG TTT TTG GAA CGC CAG AG | 20 |
| TetQ-FW | tet(Q) | TTA TAC TTC CTC CGG CAT CG | 20 |
| TetQ-RV | tet(Q) | ATC GGT TCG AGA ATG TCC AC | 20 |
| TetT-FW | tet(T) | AAGGTTTATTATATAAAAGTG | 1 |
| TetT-RV | tet(T) | AGGTGTATCTATGATATTTAC | 1 |
| TetW-FW | tet(W) | GAGAGCCTGCTATATGCCAGC | 1 |
| TetW-RV | tet(W) | GGGCGTATCCACAATGTTAAC | 1 |