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NVJ1 expression levels control the occurrence and morphology of NV junctions. (A) wt cells + empty vector; (B) nvj1-Δ cells + empty vector; (C) wt cells + PCUP1-NVJ1; (D) wt cells + PCUP1--NVJ1-GFP. Overexpression from the CUP1 promoter was achieved by adding 0.1 mM CuSO4 to growth media for 3 h. Cells were subsequently fixed and prepared for TEM as described in Material and Methods.
