FIG. 1.

Effects of CPZ and TZ on percent annexin V binding by monocyte subsets of Ficoll preparations of PBMCs. Subpopulations of the Ficoll preparation containing PBMCs from healthy donors were separately stimulated for 3 days with M. tuberculosis (H37Rv [ATCC 27294]) or with concentrations of CPZ and TZ that ranged from 0 to 0.5 mg/liter. After 3 days the cells were harvested and stained with fluorescent antibodies against alpha-beta TCR⁺, gamma-delta TCR⁺, and CD14⁺ macrophages in combination with annexin V. Cells were analyzed with an Ortho absolute flow cytometer. Results are expressed as the mean ± standard deviation percent annexin V binding in lymphocytes. Annexin V binding in excess of 10% is an indication of early apoptosis (32). The highest concentrations of CPZ and TZ that had a nominal effect on the percent annexin V binding were 0.1 mg/liter.