FIG. 4.

Inhibition of HIV-1 IN-catalyzed 3′-processing and strand transfer reactions by WM5 and WM5E. The strand transfer products migrate more slowly than the 21-mer substrate (A, darker exposure) and the 3′-processing products G, C, and L (B, lighter exposure). Lanes: 1, DNA and IN without drugs; 2, DNA alone; 3 to 10, DNA, IN, and a titration of RDS 1028 (1, 0.33, and 0.1 μM in lanes 3 to 5, respectively), WM5E (100 and 33 μM in lanes 6 and 7, respectively), and WM5 (100, 33, and 11 μM in lanes 8 to 10, respectively).