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Binding of WM5 to TAR RNA. Shown is the fraction of bound quinolone (ν) versus the TAR concentration for WM5 as inferred by fluorometric titrations (see text). Experiments were performed with a mixture of Tris-HCl (10 mM, pH 7.0), NaCl (20 mM), and Mg(ClO4)2 (1 mM) at 25°C.
