Table 1.
Junctional conductances in oocyte pairs injected with inx2 or inx2+inx3 mRNAs
| RNA injected | Number coupled/totala | Conductance (μS)b |
|---|---|---|
| H2O (—) | 0 /19 (0) | 0.00 ± 0.05 (19) |
| inx2 (10 ng) | 17 /39 (44) | 4.62 ± 1.05 (17) |
| inx2+inx3 (2+2 ng) and (5+5 ng) | 76 /78 (97) | 17.99 ± 1.58 (76) |
mRNAs were injected at the amounts indicated (ng); cells were paired and recorded electrophysiologically to determine intercellular coupling. In the inx2+inx3 experiments, both mRNAs were injected into each cell. Junctional conductance (gj) was calculated from double voltage-clamp recordings as described in Figure 5. Values are the maximum gj, recorded at transjunctional voltage steps of 10–20 mV. Note that the mean gj value for inx2 and inx2+inx3 experiments does not include noncoupled cell pairs.
a
Values in parentheses are percentages.
b
Values are means ± SEM for n cell pairs, in parentheses.