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Hydrolysis of 1.25 μM PPHPC and PPHPM/POPG (50:50, molar ratio) liposomes by bee venom (A) and human lacrimal fluid (B) sPLA2. The vesicle compositions were PPHPC with 10 μM Ca2+ (a), PPHPC with 5 mM Ca2+ (b and e), PPHPM/POPG with 10 μM Ca2+ (c), and PPHPM/POPG with 5 mM Ca2+ (d and f), in the presence (5 mol%, crosshatched) and absence (hatched) of the cationic gemini surfactant SR-1. *, P < 0.05. Each data point represents mean plus or minus standard error of the mean (n = 5).
