TABLE 2.
Sequencing results for naïve SME Cys69X, SME Cys238X, and SME Cys69X-Cys238X libraries
| SME library | Amino acid (codon)
|
|
|---|---|---|
| Cys69 | Cys238 | |
| Cys69X | Cys69 (TGC)a | |
| Ala4b (GCG) | ||
| Leu (CTC) | ||
| Pro (CCG) | ||
| Cys2 (TGC) | ||
| Thr (ACG) | ||
| Arg2 (AGG) | ||
| Arg (CGC) | ||
| Lys (AAG) | ||
| Trp (TGG) | ||
| Tyr (TAC) | ||
| Cys238X | Cys238 (TGT)a | |
| Ala2 (GCC) | ||
| Gly (GGG) | ||
| Ile (ATC) | ||
| Leu (TTC) | ||
| Pro (CCG) | ||
| Val (GTG) | ||
| Ser (TCG) | ||
| Glu2 (GAG) | ||
| Tyr (TAC) | ||
| Stop (TAG) | ||
| Cys69X-Cys238Xc | Cys69 (TGC)a | Cys238 (TGT)a |
| Trp (TGG) | Arg (AGG) | |
| Asp (GAC) | Lys (AAG) | |
| His (CAC) | Lys (AAG) | |
| Ala (GCG) | Stop (TAG) | |
| Cys (TGC) | Trp (TGG) | |
| Pro (CCC) | Asn (AAC) | |
| Val (CTC) | Asn (AAC) | |
| Cys (TGC) | Glu (GAG) | |
| Gly (GGC) | Lys (AAG) | |
| His (CAC) | Ser (AGC) | |
| Gly (GGC) | Ala (GCC) | |
| Asp (GAC) | Ala (GCC) | |
| Leu (CTC) | Leu (TTG) | |
a
The amino acid and codon found in the wild-type SME-1 gene.
b
The superscript numbers represent the numbers of clones sequenced.
c
Cys69X and Cys238X were connected by —CH2-S—S-CH2—.