TABLE 4.
Sequencing results from selection of SME Cys69X, SME Cys238X, and SME Cys69X-Cys238X libraries on plates with ampicillin, imipenem, and cefotaxime
| SME library and antibiotic used for selection | Amino acid (codon)
|
|
|---|---|---|
| Cys69 | Cys238 | |
| Cys69X | Cys69 (TGC)a | |
| Ampicillin | Cys10b (TGC) | |
| Imipenem | Cys20 (TGC) | |
| Cefotaxime | Cys10 (TGC) | |
| Cys238X | Cys238 (TGT)a | |
| Ampicillin | Cys15 (TGC) | |
| Imipenem | Cys12 (TGC) | |
| Cefotaxime | Cys12 (TGC) | |
| Cys69X-Cys238Xc | ||
| Ampicillin | Cys16 (TGC) | Cys16 (TGC) |
| Imipenem | Cys15 (TGC) | Cys15 (TGC) |
| Cefotaxime | Cys28 (TGC) | Cys28 (TGC) |
a
The amino acid and codon found in the wild-type SME-1 gene.
b
The superscript numbers represent the numbers of clones sequenced.
c
Cys69X and Cys238X were connected by —CH2-S—S-CH2—.