TABLE 2.
Synergism between ergosterol biosynthesis inhibitors and calcineurin inhibitors is FKBP12 and calcineurin dependent
Genotype (C. albicans strain) | MIC100 alone (μg/ml)a
|
MIC100 combineda,b
|
FIC indexa
|
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
CsA | FK506 | F | T | CsA/F | CsA/T | FK506/F | FK506/T | CsA/F | CsA/T | FK506/F | FK506/T | |
Wild type (SC5314) | >3.12 | >3.12 | 3.12 | >10.5 | 1.56/0.04 | 0.78/3.12 | ≤0.04/≤0.01 | 0.09/0.39 | 0.26 | 0.24 | 0.004 | 0.03 |
cnb1/cnb1 (DAY364) | >3.12 | >3.12 | ≤0.01 | 0.39 | ≤0.04/≤0.01 | ≤0.04/0.39 | ≤0.04/≤0.01 | ≤0.04/0.39 | 1.003 | 1.003 | 1.003 | 1.003 |
cnb1/cnb1 CNB1 (MCC85) | >3.12 | >3.12 | 0.78 | >12.5 | 0.78/0.04 | 0.19/3.12 | ≤0.04/≤0.01 | ≤0.04/0.78 | 0.17 | 0.15 | 0.009 | 0.03 |
CNB1-1/CNB1 (YAG237) | >3.12 | >3.12 | 3.12 | >12.5 | 1.56/0.19 | 0.78/3.12 | >3.12/3.12 | >3.12/>12.5 | 0.31 | 0.25 | 2.0 | 2.0 |
rbp1/rbp1 (YAG171) | >3.12 | >3.12 | 3.12 | >12.5 | 0.19/0.78 | 0.39/1.56 | >3.12/3.12 | >3.12/>12.5 | 0.28 | 0.12 | 2.0 | 2.0 |
F, fenpropimorph; T, terbinafine.
Combined MICs, expressed as [drug 1]/[drug 2] are the minimum concentrations of drugs 1 and 2 required to inhibit cell growth when the two drugs were used in combination.