TABLE 1.
Specific primers used for PCR and their positions
| Gene product | Primer name | 5′ primer positiona | Sequence | Product size (bp) |
|---|---|---|---|---|
| 23S rRNA | 23S-3 | 1902 | 5′-CGGCGGCCGTAACTATAACG | 1,001 |
| 23S-4 | 2902 | 5′-TTGGATAAGTCCTCGAGCTATT | ||
| HF2330 | 2331 | 5′-GTATAAGCAAGCTTAACTG | 441 | |
| HF2771 | 2770 | 5′-CAAGTTTCGTGCTTAGATG | ||
| L4 | L4-1 | −38 | 5′-TTAAGCCGGCAGTTAAAGC | 662 |
| L4-2 | +21 | 5′-CACTTAGCAAACGTTCTTG | ||
| L22 | L22-1 | −45 | 5′-CGGCAGATAAGAAAGCTAAG | 296 |
| L22-2 | +38 | 5′-TGGATGTACTTTTTGACCC |
a
23S rRNA primer positions are expressed in terms of the E. coli 23S rRNA numbering system, and those for ribosomal protein L4 and L22 bases are expressed relative to start and stop codons.