FIG. 2.

Phylogenetic trees based on sequence similarities of the genes coding for the A subunits (A) and the B subunits (B) of type II topoisomerases in gram-positive bacteria. Resistance genes of the aminocoumarin antibiotic producers are shown in boldface. The phylogeny was constructed with DNASIS for Windows, version 2 (Hitachi, San Bruno), scoring with a gap penalty of 5.0, a K-tuple of 2.0, a fixed gap penalty of 10.0, and a floating gap penalty of 10.0. The number of top diagonals and window size were both set 5. Bacterial strains: Sta., Staphylococcus; Bac., Bacillus; Clo., Clostridium; Myc., Mycobacterium; Cor., Corynebacterium; Str., Streptomyces.