. 2003 Mar;47(3):869–877. doi: 10.1128/AAC.47.3.869-877.2003

TABLE 2.

Growth of S. lividans TK24 harboring resistance genes from S. spheroides (Ss) and S. rishiriensis (Sr)

Gene(s)	Growth^a with novobiocin at (μg/ml):							Novobiocin MIC (μg/ml)	Growth with coumermycin A₁ at (μg/ml):						Coumermycin A₁ MIC (μg/ml)
Gene(s)	0	50	100	250	500	600	750	Novobiocin MIC (μg/ml)	0	50	100	250	300	500	Coumermycin A₁ MIC (μg/ml)
Control (pUWL201)	+++++	+	0	0	0	0	0	100	+++++	0	0	0	0	0	50
Ss gyrB^R	+++++	+++++	+++++	+++++	++++	+++	+	>750	+++++	+++++	++++	+++	++	0	500
Sr gyrB^R	+++++	+++++	+++++	+++++	++++	+++	+	>750	+++++	+++++	++++	+++	++	0	500
Sr parY^R	+++++	+++++	+++++	++++	0	0	0	500	+++++	+++++	+++	+	0	0	300
Sr gyrB^R + parY^R	+++++	+++++	+++++	+++++	++++	+++	+	>750	+++++	+++++	++++	+++	++	0	500
Ss novA	+++++	+++++	++	+	0	0	0	500	+++++	++++	+	0	0	0	250
Sr couR5	+++++	++++	++	0	0	0	0	250	+++++	++++	+	0	0	0	250

+++++, more than 3,000 colonies; ++++, 1,000 to 3,000 colonies; +++, 500 to 1,000 colonies; ++, 100 to 500 colonies; +, 10 to 100 colonies; 0, no growth observed. MIC, minimum concentration causing complete growth inhibition under the conditions of this experiment.