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. 2003 Mar;47(3):869–877. doi: 10.1128/AAC.47.3.869-877.2003

TABLE 3.

Alignment of amino acids involved in aminocoumarin binding by E. coli gyrase, with corresponding positions of type II topoisomerase B subunits of different bacteriaa

Species Protein Size (aa) Position
E. coli GyrB 804 Val 43 Asn 46 Glu 50 Asp 73 Gly 77 Ile 94 Val 120 Arg 136 Gly 164 Thr 165
    Novobiocin     interaction + + + + − − − + − −
    Clorobiocin     interaction + + + + + − − + − +
Staphylococcus aureus GyrB 644 Ile 51 Asn 54 Glu 58 Asp 81 Gly 85 lle 102 Ser 128 Arg 144 Gly 172 Thr 173
S. pneumoniae GyrB 648 Ile 50 Asn 53 Glu 57 Asp 80 Gly 84 Val 101 Ser 127 Lys 143 Gly 171 Thr 172
S. coelicolor GyrB 687 Val 70 Asn 73 Glu 77 Asp 100 Gly 104 Val 121 Val 147 Thr 163 Gly 191 Thr 192
S. spheroides GyrBR 677 Leu 63 Asn 66 Glu 70 Asp 93 Gly 97 Val 114 Leu 140 Thr 156 Gly 184 Thr 185
S. rishiriensis GyrBR 677 Leu 63 Asn 66 Glu 70 Asp 93 Gly 97 Val 114 Leu 140 Thr 156 Gly 184 Thr 185
S. roseochromogenes GyrBR 677 Leu 63 Asn 66 Glu 70 Asp 93 Gly 97 Val 114 Leu 140 Thr 156 Gly 184 Thr 185
S. coelicolor ParY 707 Ile 60 Asn 63 Glu 67 Asp 90 Gly 94 Val 111 Ala 137 Leu 153 Gly 197 Thr 198
S. rishiriensis ParYR 702 Ile 56 Asn 59 Glu 63 Asp 86 Gly 90 Ala 107 Ala 132 Arg 148 Gly 192 Thr 193
S. roseochromogenes ParYR 702 Ile 56 Asn 59 Glu 63 Asp 86 Gly 90 Ala 107 Ala 132 Arg 148 Gly 193 Thr 194
a

Resistance proteins of the aminocoumarin producers are shown in bold face. Boldface amino acids are positions of known mutations conferring resistance to aminocoumarins in E. coli, Staphylococcus aureus (28), or Streptococcus pneumoniae (21). The interaction of E. coli gyrase with aminocoumarins has been described (16, 32).