Figure 1.

(A) Flow cytometry of Caco-2 and SW-620 cells demonstrating integrin α5 expression (gray areas). (B) Flow cytometry of Caco-2 cell lines, A105 and A9, stably transfected with integrin α5 (gray areas). The cells were stained with an integrin α5 mAb and FITC-conjugated goat anti-mouse antibodies or with FITC-labeled goat anti-mouse antibodies alone (controls; unshaded areas). The ordinate represents the number of cells counted (in thousands) and the abscissa the fluorescein intensity. (C) Immunoblots of integrin α5 immunoprecipitates from Caco-2 cells stably transfected with vector alone (B6 and B7) and integrin α5. A single ∼120-kDa band (arrow), corresponding to the heavy chain of integrin α5, was obtained under reducing conditions. (D) Integrin α3, α4, and αV subunit expression in the control and integrin α5–transfected cells determined by flow cytometry. The transfectants were stained with integrin α3, α4, and αV mAbs followed by FITC-conjugated goat anti-mouse antibodies. For each integrin subunit, the fluorescence intensity was expressed as a percentage of the controls (stained with FITC-conjugated goat anti-mouse antibodies alone).