Figure 6.

Shc (A), EGFR (B), and phosphotyrosine (C) immunoblots were performed on Shc immunoprecipitates from control and integrin α5–transfected cells grown for 2 d on uncoated (P) or fibronectin-coated (Fn) plastic. Shc immunoprecipitation was performed on the different lysates that had been normalized for total protein. Each of the immunoprecipitates was then divided into three aliquots, which were used to generate the different immunoblots in A, B, and C. Panel A shows that equal amounts of Shc were immunoprecipitated from the different cell lysates.