TABLE 1.
E. coli strains useda
| Strain | Relevant genotype | Construction or reference |
|---|---|---|
| HN818 | acrA::Tn903kanr | 31 |
| 1358 (LE234) | F−metB argE ilv tna | 33 |
| 1359 (LE316) | 1358 gyrB234 (Cour, Ts) | 33 |
| E3426 | MG1655 recD::Tn10 | S. Kustu (constructed from reference 9) |
| 2819 | recD parER132C:cat | Transformation of E3426 with linear DNA, Chlr |
| 2822 | 1358 parER132C:cat | P1(2819) × 1358, Chlr |
| 2824 | 1359 parER132C:cat | P1(2819) × 1359, Chlr |
| 2817 | 1358 acrA::Tn903kanr | P1(HN818) × 1358, Kanr |
| 2818 | 1359 acrA::Tn903kanr | P1(HN818) × 1359, Kanr |
| 2834 | 2822 acrA::Tn903kanr | P1(HN818) × 2822, Kanr |
| 2832 | 2824 acrA::Tn903kanr | P1(HN818) × 2824, Kanr |
a
Abbreviations: Cou, coumermycin; Chl, chloramphenicol; Kan, kanamycin; r, resistant; Ts, temperature sensitive; P1, source of transducing lysate. Constructions are shown as method of transformation and selection or as P1 donor × recipient and method of selection.