Table 2.
The binding of IMV and EEV at different temperatures
| Percentage of bindinga
|
||||
|---|---|---|---|---|
| 1 h | 2 h | 3 h | 4 h | |
| IMV | ||||
| 4°C | 1.9 | 2.7 | 4.6 | 4.2 |
| 15°C | 4.6 | 6.7 | 9.0 | 8.9 |
| 18°C | 2.8 | 3.8 | 7.6 | 9.0 |
| 22°C | 2.9 | 4.5 | 10.6 | 10.8 |
| EEV | ||||
| 4°C | 2.0 | 3.6 | 5.7 | 7.3 |
| 15°C | 4 | 4.8 | 7.0 | 7.9 |
| 18°C | 3.4 | 3.2 | 5.9 | 9.3 |
| 22°C | 3.1 | 8.9 | 12.8 | 17.7 |
a
100 000 cpm of 35S-labeled IMV and EEV preparations were bound for the indicated times and indicated temperatures to monolayers of HeLa cells at a MOI of 1. Percentage of binding was calculated by dividing the cell-associated counts by the total counts. The values are the average of duplicates and from two independent experiments.