Table 5.
The effect of overexpression of dominant-negative or activated rho proteins and of full-length ezrin or ezrin 1-309 on IMV and EEV entrya
| IMV
|
EEV
|
|||||
|---|---|---|---|---|---|---|
| Not transf.b | Transf.c | % of Contr.d | Not transf.b | Transf.c | % of Contr.d | |
| V14RhoA | 32.2 ± 10.2 | 9.4 ± 65.0 | 29 | 39.2 ± 12.6 | 35.3 ± 11.4 | 90 |
| N19RhoA | 42.5 ± 15.9 | 40.9 ± 19.8 | 100 | 46.1 ± 28.0 | 46.2 ± 22.0 | 100 |
| L61rac1 | 32.6 ± 10.8 | 64.6 ± 25.9 | 200 | 39.2 ± 14.3 | 43.7 ± 15.8 | 110 |
| N17rac1 | 35.1 ± 11.8 | 20.2 ± 11.1 | 58 | 37.8 ± 15.5 | 40.5 ± 15.3 | 107 |
| L61CDC42 | 35.6 ± 14.0 | 33.9 ± 14.6 | 95 | 43.1 ± 20.7 | 41.8 ± 18.4 | 97 |
| N17CDC42 | 33.3 ± 14.1 | 35.1 ± 12.3 | 105 | 40.4 ± 16.7 | 39.6 ± 16.8 | 100 |
| Ezrin-fl | 36.1 ± 17.2 | 36.1 ± 17.4 | 100 | 46.3 ± 16.0 | 45.8 ± 16.1 | 100 |
| Ezrin-N | 36.9 ± 17.1 | 54.4 ± 21.0 | 147 | 48.4 ± 27.8 | 50.5 ± 25.8 | 105 |
a
IMV infection was at MOI 30 and EEV at MOI 10.
b
Average number of cores and S.D.s in nontransfected HeLa cells. N = 25 ± S.D.s.
c
Average number of cores and S.D.s in transfected cells (anti-myc or anti-P5D4 positive) with activated rhoA (V14 rhoA), rac1 (L61rac1), CDC42 (L61CDC42), dominant-negative rhoA (N19rhoA), rac1 (N17rac1), and CDC42 (N17CDC42), and full-length ezrin (ezrin-fl) or ezrin 1-309 (ezrin-N). N = 25.
d
% of contr. indicates the percentage of inhibition or stimulation of entry on overexpression of the respective proteins compared to untransfected cells.