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. 2003 Jan 24;3:3. doi: 10.1186/1471-2415-3-3

Figure 3.

Figure 3

Representative confocal optical sections from alphaA/BKO (A-J) and wild type (K-T) lenses. All optical sections were generated using a 63 × /1.4 objective on a Zeiss LSM 410. The red color represents DiI staining (membrane), while the green color represents SYTOX green staining (DNA). (A-E) are from 5 wk alphaA/BKO lenses. (F-J) are from 54 wk alphaA/BKO lenses. (K-O) are from 5 wk wild type lenses. (P-T) are from 46 wk wild type lenses. (A, F, K, and P) are anterior central cortex and epithelium. (B, G, L, and Q) are equatorial bow region. (C, H, M, and R) are embryonic/fetal nucleus. (D, I, N, and S) are deep anterior cortex. (E, J, O, and T) are posterior subcapsular region. (*) indicates areas devoid of cellular material (B and G). Arrow indicates posterior suture (O). Arrowheads indicate representative nuclei stained with SYTOX green (A, B, E, F, G, I, K, L, P, and Q).