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. 2000 Aug;11(8):2513–2528. doi: 10.1091/mbc.11.8.2513

Figure 4.

Figure 4

Effect of RhoGV12, Rac1V12, Cdc42HsV12, and RhoAV14 expression on myogenesis. (A) Protein extracts from parental L6 (a and b), L6 Rac1V12 (c), L6 Cdc42HsV12 (d), and L6 RhoAV14 (e) (100 μg/well) collected at the indicated periods (P, proliferative; D1, D2, D3, and D4, 1 to 4 d after addition of differentiation medium) were immunoblotted with anti-Rac1 (a and c), anti-α-tubulin (b), anti-Myc (d), or anti-HA (e) antibody as described in MATERIALS AND METHODS. (B) Proliferating parental L6 and L6 RhoGV12, L6 Rac1V12, and L6 Cdc42HsV12 myoblasts were grown to ∼80% and then induced to differentiate in DM. Shown are phase-contrast images of parental L6 myoblasts (a–c), L6 RhoGV12 (d–f), L6 Rac1V12 (g–i), L6 Cdc42HsV12 (j–l), and L6 RhoAV14 cells (m–o). Panels a, d, g, j, and m, cells in proliferating medium; panels b, e, h, k, and n, 1 d after addition of DM; panels c, f, i, l, and o, 3 d after addition of DM. For each panel, cells shown are representative of more than 100 observed cells. Bar, 10 μm.