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. 2000 Aug;11(8):2513–2528. doi: 10.1091/mbc.11.8.2513

Figure 8.

Figure 8

Myf5 translocation to a cytoplasmic compartment in RhoG-, Rac1-, and Cdc42Hs-expressing cells and after JNK activation. (A) Protein extracts from parental L6, L6 RhoGV12, L6 Rac1V12, and L6 Cdc42HsV12 (100 μg/well) were immunoblotted with anti-Myf5 antibody as described in MATERIALS AND METHODS. (B) Parental L6 (a and b), L6 RhoAV14 (c), L6 RhoGV12 (d), L6 Rac1V12 (e), and L6 Cdc42HsV12 (f) were fixed and stained for Myf5 expression. Panels a, c, d, e, and f, cells in proliferating medium; panel b, 1.5 d after the addition of DM. For each panel, cells shown are representative of five independent experiments with more than 100 observed cells. Bar, 10 μm. (C) Parental L6 (a) and L6 RhoAV14 (b) were treated with anisomycin (50 nM) for 24 h and then fixed and stained for Myf5 localization. L6 myoblasts were transfected with constructs expressing FLAG-tagged MKK7 or HA-tagged MKK3. Twenty-four hours after transfection, cells were fixed and processed for FLAG (c, inset) or HA epitope detection (d, inset) and for Myf5 localization (c and d). For each panel, cells are representative of three independent experiments.