Table 1.
Amount of syntaxin 5 and ribophorin in reconstituted tER
| Protein | Networks (n) | Gold particles (n) | Surface area of SERa (%) | Labeling over SER (%) | Concentration in SERb |
|---|---|---|---|---|---|
| Syntaxin 5 | 41 | 401 | 73.9 | 79.8 | 1.38X |
| Ribophorin II | 11 | 462 | 75.7 | 59.5 | 0.47X |
a
SER corresponds to the smooth ER tubules of the reconstituted tER.
b
To calculate concentrations, percent antigen labelling densities were determined for the rough and smooth ER compartments, and these values were compared to the relative amounts of surface areas occupied by each compartment as described previously (Lavoie et al., 1999).