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Stimulatory effects of the expression of a dominant negative mutant of SHP-2 on the formation of stress fibers and focal adhesions in MDCK cells. Wild-type MDCK cells (WT) (A and C) and MDCK-SHP-2-C/S cells (SHP-2-C/S) (B and D) were stained with rhodamine-conjugated phalloidin (A and B) or the anti-vinculin mAb (C and D) and then analyzed by confocal microscopy at the basal level. The results shown are representative of three independent experiments. Bar, 10 μm.
