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. 2000 Aug;11(8):2565–2575. doi: 10.1091/mbc.11.8.2565

Figure 5.

Figure 5

Reversal by Y-27632 of the inhibition of HGF/SF-induced cell scattering in MDCK-SHP-2-C/S cells. Wild-type MDCK cells (WT) (Aa–Ad) and MDCK-SHP-2-C/S cells (SHP-2-C/S) (Ba–Bd) were incubated with no Y-27632 (Aa, Ac, Ba, and Bc) or 10 μM Y-27632 (Ab, Ad, Bb, and Bd). At 30 min after the addition of Y-27632, the cells were stimulated with no HGF/SF (Aa, Ab, Ba, and Bb) or 10 ng/ml HGF/SF (Ac, Ad, Bc, and Bd). At 18 h after HGF/SF stimulation, the cells were fixed, stained with rhodamine-conjugated phalloidin, and analyzed by confocal microscopy. Confocal images are shown at the basal level. The results shown are representative of three independent experiments. Bar, 10 μm.