Figure 6.

Expression of wild-type Vav2 and its mutants. (A) Structural domains of Vav2 are schematically illustrated at the top, and wild-type Vav2 cDNA and six Vav2 mutants are represented by thick lines below. Numbers indicate amino acid residues of the N and C termini of each domain. LR, leucine-rich domain; AD, acid-rich domain; CR, cystein-rich domain; SH3, src homology-3; ×, positions of point mutations with amino acid numbers. (B) Various pCIneo-myc plasmids encoding wild-type Vav2 and each Vav2 mutant were transiently transfected into COS-7 cells. At 48 h after transfection, the cell lysates were prepared from each transfected cell and subjected to immunoblotting with the anti-Myc mAb.